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Recombinant human BAFF / CD257 Protein, Fc Tag, 100µg  

Recombinant human BAFF / CD257 Protein, Fc Tag, 100µg

Recombinant Human BAFF / CD257 Protein (Ala134-Leu285), produced in human 293 cells (HEK293), N-Fc tag

Synonym
Recombinant human protein, TNFSF13B, BAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK, TNFSF20, ZTNF4

More details

BAF-H4268-100

Availability: within 7 days

420,00 €

Background
B-cell activating factor (BAFF), also known as tumor necrosis factor ligand superfamily member 13B , TNFSF13B, BAFF, B Lymphocyte Stimulator (BLyS) , cluster of differentiation 257 (CD257), DTL, TNF- and APOL-related leukocyte expressed ligand (TALL-1), THANK, TNFSF20, ZTNF4, and is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it. These receptors are expressed mainly on mature B lymphocytes (TACI is also found on a subset of T-cells and BCMA on plasma cells). TACI binds worst since its affinity is higher for a protein similar to BAFF, called a proliferation-inducing ligand (APRIL). BCMA displays an intermediate binding phenotype and will work with either BAFF or APRIL to varying degrees. Signaling through BAFF-R and BCMA stimulates B lymphocytes to undergo proliferation and to counter apoptosis. All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors[1], although BAFF has been known to be active as either a hetero- or homotrimer.[2] BAFF acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells.

Source
Recombinant Human BAFF /CD257 Protein, With N-Fc Tag (rhBAFF-Fc) Ala134-Leu285(Accession # AAH20674.1) was produced in human 293 cells (HEK293)

Molecular Characterization
rhBAFF-Fc fused with Fc fragment of human IgG1 at the N-terminal and has a calculated MW of 44.5 kDa. DTT-reduced protein migrates as 45-55 kDa polypeptide in SDS-PAGE resulting from glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhBAFF-Fc by the LAL method.

Purity
>96% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0.  Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Oren,D.A., et al., 2002, Nat. Struct. Biol. 9 (4): 288–292.
(2) Daridon C, er al.,2008, Autoimmun Rev 7 (4): 267–71