Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human CXCR4 Protein, GST tag, 50µg  

Recombinant human CXCR4 Protein, GST tag, 50µg

Recombinant Human CXCR4 Protein, Met 1 - Ser 46, produced in E.coli cells, GST Tag

Synonym
Recombinant Human Protein, CXCR4, CD184, Fusin, D2S201E, FB22, HM89, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM

More details

CX4-H5138-050

Availability: within 7 days

312,00 €

Background
C-X-C chemokine receptor type 4 is also known as fusin or CD184 (cluster of differentiation 184), CXCR4, CD184, D2S201E, FB22, HM89, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R or WHIM. CXCR-4 is an alpha-chemokine receptor specific for stromal-derived-factor-1 (SDF-1 also called CXCL12), a molecule endowed with potent chemotactic activity for lymphocytes. This receptor is one of several chemokine receptors that HIV isolates can use to infect CD4+ T cells. HIV isolates that use CXCR4 are traditionally known as T-cell tropic isolates. Typically, these viruses are found late in infection. It is unclear as to whether the emergence of CXCR4 using HIV is a consequence or a cause of immunodeficiency.CXCR4 is upregulated during the implantation window in natural and hormone replacement therapy cycles in the endometrium, producing, in presence of a human blastocyst, a surface polarization of the CXCR4 receptors suggesting that this receptor is implicated in the adhesion phase of human implantation. SDF-1 and CXCR4 were believed to be a relatively "monogamous" ligand-receptor pair (other chemokines tend to use several different chemokine receptors in a fairly "promiscuous" manner). Recent evidence demonstrates ubiquitin is also a natural ligand of CXCR4. Chronic exposure to THC increased T lymphocyte CXCR4 expression on both CD4+ and CD8+ T lymphocytes. Drugs that block the CXCR4 receptor appear to be capable of "mobilizing" hematopoietic stem cells into the bloodstream as peripheral blood stem cells.

Source
Recombinant Human CXCR4 Protein, With N-GST Tag (rhCXCR4) Met 1 - Ser 46 (Accession # AAH20968.1) was produced in E.coli cells.

Molecular Characterization
rhCXCR4, fused with GST tag at the N-terminus, has a calculated MW of 32.2 kDa. The predicted N-terminus is Met 1. DTT-reduced Protein migrates as 33 kDa.

Endotoxin
Less than 1.0 EU per μg of the rhCXCR4 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with 10 mM reduced glutathione. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C).

References

(1) Saini V, Marchese A, Majetschak M, 2010, J. Biol. Chem. 285 (20): 15566–76.
(2) LeCapitaine NJ,et al., 2011, J Neuroimmune Pharmacol., 6(4): 540-5.