Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human CD34 Protein, Fc tag, 100µg  

Recombinant human CD34 Protein, Fc tag, 100µg

Recombinant Human CD34 Protein (Ser32-Thr290), produced in human 293 cells (HEK293), Fc tag

Synonym
Recombinant human protein, RP11-328D5.2, CD34

More details

CD4-H5255-100

Availability: within 7 days

378,00 €

Background
CD34 molecule is a cluster of differentiation molecule present on certain cells within the human body. It is a cell surface glycoprotein and functions as a cell-cell adhesion factor. It may also mediate the attachment of stem cells to bone marrow extracellular matrix or directly to stromal cells.[1-2] As a ~ 110 kDa monomeric cell surface antigen, CD34 is highly glycosylated with nine potential N-linked and numerous potential O-linked glycosylation sites in its extracellular domain. The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue [3]. CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. It is expressed on lymph node endothelia whereas the L-selectin to which it binds is on the T cell.[4-5]. It was indicated that CD34 is a phosphorylation target for activated PKC, and couples to the hematopoietic adapter protein CrkL, which were involved in CD34 signaling pathways. CD34 is abberantly expressed in many kinds of tumors and is implicated in leukemogenesis.

Source
Recombinant Human CD34, Fc Tag (CD4-H5255) is expressed from human 293 cells (HEK293). It contains AA Ser 32 - Thr 290 (Accession # P28906-1).

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 53.6 kDa. The protein migrates as 100 kDa and 116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD34-Fc by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

[1]Simmons DL, et al., 1992, J. Immunol. 148 (1): 267–71.
[2]Satterthwaite AB, et al., 1992, Genomics 12 (4): 788–94.
[3]Nielsen JS, McNagny KM., 2008, J of Cell Science 121 (Pt 22): 3682–3692.
[4]Berg EL, et al., 1998, Am. J. Pathol. 152 (2): 469–77.
[5]SSuzawa K, et al., 2007, Am. J. Gastroenterol. 102 (7): 1499–509.