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Recombinant human IL4R /CD124 Protein, His Tag, 1mg  

Recombinant human IL4R /CD124 Protein, His Tag, 1mg

Recombinant Human IL4R /CD124 Protein, Met 26 - His 232 was produced in human 293 cells (HEK293), His Tag

Synonym
Recombinant Human Protein, IL4R, CD124, IL4RA

More details

ILR-H5221-1K

Availability: within 7 days

1 800,00 €

Background
IL-4 is a pleiotropic cytokine produced by activated Th2 cells and mast cells, and plays a pivotal role in immune responses. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells. Hematopoietic cellular responses to IL-4 are mediated by a high affinity receptor complex comprised of the 140 kDa IL4Rα (CD124)subunit and the 70 kDa common cytokine γc chain (CD132). Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL13Ra1 to form the type II receptor which is responsive to both IL4 and IL13. Interleukin-4 receptor has been shown to interact with SHC1.[1-5]

Source
Recombinant Human IL4R /CD124 Protein (rhIL4R) Met 26 - His 232(Accession # NP_000409.1)was produced in human 293 cells (HEK293).

Molecular Characterization
rhIL4R, fused with a C-terminal 6×His tag, has a calculated MW of 24.5 kDa expressed. The predicted N-terminus is Met 26. Protein migrates as 35-45 kDa in reduced SDS-PAGE.

Endotoxin
Less than 1.0 EU per μg of the rhIL4R by the LAL method.

Purity
>95% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Immobilized Human IL-4 R alpha, His Tag (Cat. No. ILR-H5221) at 5μg/mL (100 μL/well) can bind Biotinylated Human IL-4, His Tag with a linear range of 1.95-15.6 ng/mL (QC tested)..

References

(1) Ikizawa, K., et al., 2000, Biochem. Biophys. Res. Commun. (UNITED STATES) 268 (1): 54–9.
(2) Kashiwada, M., et al., 2001, J. Immunol. (United States) 167 (11): 6382–7.
(3) Hage T., et al., 1999, Cell 97 (2): 271–81.
(4) Gessner,A. et al., 2000, Immunobiology. 201 (3-4): 285-307.
(5) Wills-Karp, M., F.D.Finkelman, Sci Signal. 2008;1(51):pe55. doi: 10.1126/scisignal.1.51.pe55.