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Recombinant human IL-2 R beta /CD122 Protein, His tag, 100µg  

Recombinant human IL-2 R beta /CD122 Protein, His tag, 100µg

Recombinant Human IL-2 R beta /CD122 Protein, Ala 27 - Asp 239, produced in human 293 cells (HEK293), His tag

Synonym
Recombinant Human Protein, IL2RB, RP5-1170K4.6, CD122, P70-75

More details

CD2-H5221-100

Availability: within 7 days

462,00 €

Background
Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to a cytokine called IL-2. The IL-2R is made up of 3 subunits - α (CD25), β (CD122) and γ (CD132) - non-covalently associating. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ-chain, along with the β subunit. CD122 also known as IL2R beta, is a member of the type I cytokine receptor family. CD122 is the receptor for interleukin-2 and is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.

Source
Human IL-2 R beta, His Tag (SPR verified) (CD2-H5221) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (Accession # NP_000869.1).
Predicted N-terminus: Ala 27

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.4 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD122 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Human IL-2 R beta, His Tag (Cat. No. CD2-H5221) captured on CM5 chip via anti-His antibody, can bind Human IL-2, Native (Cat. No. IL2-H4113) with an affinity constant of 0.525 μM as determined in a SPR assay (Biacore T200) (QC tested).
Human IL-2 R beta, His Tag (Cat. No. CD2-H5221) captured on CM5 chip via anti-His antibody, can bind Human IL-15, Native (Cat. No. IL5-H4117) with an affinity constant of 21.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

References

(1) Mulloy J.C., et al., 1996, J. Virol. 70:3599-3605.
(2) Lindholm C.K., 2002, Biochem. Biophys. Res. Commun. 296:929-936.