Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human IL-2 R beta /CD122 Protein, His tag, 500µg  

Recombinant human IL-2 R beta /CD122 Protein, His tag, 500µg

Recombinant Human IL-2 R beta /CD122 Protein, Ala 27 - Asp 239, produced in human 293 cells (HEK293), His tag

Synonym
Recombinant Human Protein, IL2RB, RP5-1170K4.6, CD122, P70-75

More details

CD2-H5221-500

Availability: within 7 days

1 600,00 €

Background
Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to a cytokine called IL-2. The IL-2R is made up of 3 subunits - α (CD25), β (CD122) and γ (CD132) - non-covalently associating. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ-chain, along with the β subunit. CD122 also known as IL2R beta, is a member of the type I cytokine receptor family. CD122 is the receptor for interleukin-2 and is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.

Source
Recombinant Human IL-2 R beta /CD122 Protein (rhCD122) Ala 27 - Asp 239 (Accession # NM_000878.2) was produced in human 293 cells (HEK293).

Molecular Characterization
rhCD122, fused with 6×His tag at the C-terminus, has a calculated MW of 25.4 kDa. The predicted N-terminus is Ala 27. DTT-reduced Protein migrates as 40 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD122 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Mulloy J.C., et al., 1996, J. Virol. 70:3599-3605.
(2) Lindholm C.K., 2002, Biochem. Biophys. Res. Commun. 296:929-936.