Recombinant human MME /CD10 Protein, Fc Tag, 1mg

Background
Cluster of differentiation 10 (CD10), membrane metallo-endopeptidase, neutral endopeptidase (NEP), Neprilysin, and common acute lymphoblastic leukemia antigen (CALLA), is a 90-110-kDa type II transmembrane glycoprotein normally expressed by a variety of tissues, including epithelial cells of the prostate, kidney, intestine, endometrium, adrenal glands, and lung. This zinc-dependent metalloprotease enzyme cleaves peptide bonds on the amino side of hydrophobic residues and inactivates a variety of physiologically active secreted peptides. CD20 is thought to be the rate-limiting degrading enzyme of amyloid β peptide (Aβ) whose abnormal misfolding and aggregation in neural tissue has been implicated in the development of Alzheimer\'s disease (AD). CD10 is also identified as the common acute lymphoblastic leukemia antigen (CALLA) present on leukemic cells of pre-B phenotype, and thus serves as the most important biomarker in the diagnosis of human acute lymphocytic leukemia (ALL). [1-4]

Source
Recombinant Human Neprilysin, Fc Tag (MME-H526a) is expressed from human 293 cells (HEK293). It contains AA Tyr 52 - Trp 750 (Accession # NP_000893.2).

Molecular Characterization
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 106.8 kDa. The protein migrates as 106-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU endotoxin per μg rhCD10 by the LAL method.

Purity
>95% purity as determined by SDS-PAGE of reduced rhCD10.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Serum neprilysin levels are elevated in preeclampsia"
Tüten, Malik, Gök et al
Taiwan J Obstet Gynecol (2021) 60 (5), 869-873
(2) "Aβ31-35 Decreases Neprilysin-Mediated Alzheimer's Amyloid-β Peptide Degradation"
Leite, Lete, Fowler et al
ACS Chem Neurosci (2021)
(3) "Multi-Antigen Imaging Reveals Inflammatory DC, ADAM17 and Neprilysin as Effectors in Keloid Formation"
Rath, Pitiot, Kirr et al
Int J Mol Sci (2021) 22 (17)
Showing 1-3 of 6148 papers.
Please refer to product data sheet.