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Recombinant human VCAM1 / CD106 Protein, His Tag, 2x500µg  

Recombinant human VCAM1 / CD106 Protein, His Tag, 2x500µg

Recombinant Human VCAM1 /CD106 Protein, Phe 25 - Pro 697, produced in human 293 cells (HEK293), His Tag

Synonym
Recombinant Human Protein, VCAM1, CD106, L1CAM, INCAM-100

More details

VC1-H5224-1K

Availability: within 7 days

1 500,00 €

Background
Vascular cell adhesion protein 1 (VCAM1) also known as CD106, INCAM-100 and L1CAM, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. VCAM1 / CD106 contains 7 Ig-like C2-type (immunoglobulin-like) domains. CD106 / VCAM-1 is expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. L1CAM / VCAM-1 is Important in cell-cell recognition and appears to function in leukocyte-endothelial cell adhesion. CD106 / VCAM1 interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1 / VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. INCAM-100 / VCAM1 binds to ECMV-D capsid proteins and acts as a receptor for this virus.

Source
Recombinant Human VCAM1 /CD106 Protein (rhVCAM1) Phe 25 - Pro 697 (Accession # NP_001069) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 75 kDa. The protein migrates as 80-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhVCAM1 by the LAL method.

Purity
>92% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Osborn L., et al., 1992, J. Exp. Med. 176:99-107.
(2) Zhang Z., et al., 2004, Protein Sci. 13:2819-2824.