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Recombinant human IL2RG /CD132 Protein, Fc Tag, 100µg  

Recombinant human IL2RG /CD132 Protein, Fc Tag, 100µg

Recombinant Human IL2RG /CD132 Protein, Leu 23 - Asn 254,  produced in human 293 cells (HEK293), Fc Tag

Synonym
Recombinant Human Protein, IL2RG, CD132, CIDX, IMD4, P64, SCIDX, gammaC

More details

ILG-H5256-100

Availability: within 7 days

326,00 €

Background
IL-2R is a heterotrimeric protein binds and responds to the cytokine IL-2. Three distinct chains of IL-2R, termed as α, β and γ, which are non-covalently associated are identified. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ chain, along with the β subunit. The α chain of the IL-2R can bind to the β chain before receptor interaction with IL-2. The γ chain alone has a very weak affinity for IL-2, but after the ligand is bound to the α/β heterodimer, the γ chain becomes recruited to the complex to form a very stable macromolecular quaternary ligand/receptor complex. Interleukin-2 receptor subunit gamma (IL2RG), also known as cytokine receptor common subunit gamma, CD antigen CD132, gammaC, p64, which belongs to the type I cytokine receptor family or type 5 subfamily. IL2RG is located on the surface of immature blood-forming cells in bone marrow. Defects in IL2RG are the cause of severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell-negative (XSCID).

Source
Recombinant Human IL2RG /CD132 Protein, Fc Tag (ILG-H5256) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Asn 254 (Accession # AAH14972).
Predicted N-terminus: Leu 23

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 53.5 kDa. The protein migrates as 75-96 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhIL2RG Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Improvement of Bronchial Immune Hypersensitivity Reaction Using Extracts from Chrysanthemum morifolium Ramatuelle and Scutellaria baicalensis Georgi"
Cho, Park, Choi
Evid Based Complement Alternat Med (2021) 2021, 3173823
(2) "Guanylin ligand protects the intestinal immune barrier by activating the guanylate cyclase-C signaling pathway"
Xing, Zhang, Liu et al
Acta Histochem (2021) 124 (1), 151811
(3) "Effect of a dietary supplement on the reduction of lymphedema-progression in mouse tail-cut model"
Bhusan Tripathi, Pandey, Mishra et al
Eur Rev Med Pharmacol Sci (2021) 25 (1 Suppl), 56-66
Showing 1-3 of 1361 papers.