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Recombinant human IL2RG /CD132 Protein, Fc Tag, 100µg  

Recombinant human IL2RG /CD132 Protein, Fc Tag, 100µg

Recombinant Human IL2RG /CD132 Protein, Leu 23 - Asn 254,  produced in human 293 cells (HEK293), Fc Tag

Synonym
Recombinant Human Protein, IL2RG, CD132, CIDX, IMD4, P64, SCIDX, gammaC

More details

ILG-H5256-100

Availability: within 7 days

300,00 €

Background
IL-2R is a heterotrimeric protein binds and responds to the cytokine IL-2. Three distinct chains of IL-2R, termed as α, β and γ, which are non-covalently associated are identified. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ chain, along with the β subunit. The α chain of the IL-2R can bind to the β chain before receptor interaction with IL-2. The γ chain alone has a very weak affinity for IL-2, but after the ligand is bound to the α/β heterodimer, the γ chain becomes recruited to the complex to form a very stable macromolecular quaternary ligand/receptor complex. Interleukin-2 receptor subunit gamma (IL2RG), also known as cytokine receptor common subunit gamma, CD antigen CD132, gammaC, p64, which belongs to the type I cytokine receptor family or type 5 subfamily. IL2RG is located on the surface of immature blood-forming cells in bone marrow. Defects in IL2RG are the cause of severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell-negative (XSCID).

Source
Recombinant Human IL2RG /CD132 Protein, Fc Tag (ILG-H5256) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Asn 254 (Accession # AAH14972).
Predicted N-terminus: Leu 23

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 53.5 kDa. The protein migrates as 75-96 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhIL2RG Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Kondo M., et al., 1993, Science 262:1874-1877.
(2) Russell S.M., et al., 1993, Science 262:1880-1883.
(3) Noguchi M., et al., 1993, Science 262:1877-1880.