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Recombinant human IL2RG /CD132 Protein, Fc Tag, 100µg  

Recombinant human IL2RG /CD132 Protein, Fc Tag, 100µg

Recombinant Human IL2RG /CD132 Protein, Leu 23 - Asn 254,  produced in human 293 cells (HEK293), Fc Tag

Synonym
Recombinant Human Protein, IL2RG, CD132, CIDX, IMD4, P64, SCIDX

More details

ILG-H5256-100

Availability: within 7 days

300,00 €

Background
IL-2R is a heterotrimeric protein binds and responds to the cytokine IL-2. Three distinct chains of IL-2R, termed as α, β and γ, which are non-covalently associated are identified. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ chain, along with the β subunit. The α chain of the IL-2R can bind to the β chain before receptor interaction with IL-2. The γ chain alone has a very weak affinity for IL-2, but after the ligand is bound to the α/β heterodimer, the γ chain becomes recruited to the complex to form a very stable macromolecular quaternary ligand/receptor complex. Interleukin-2 receptor subunit gamma (IL2RG), also known as cytokine receptor common subunit gamma, CD antigen CD132, gammaC, p64, which belongs to the type I cytokine receptor family or type 5 subfamily. IL2RG is located on the surface of immature blood-forming cells in bone marrow. Defects in IL2RG are the cause of severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell-negative (XSCID).

Source
Recombinant Human IL2RG /CD132 Protein, with C-Fc Tag (rhIL2RG Fc Chimera) Leu 23 - Asn 254 (Accession # AAH14972) was produced in human 293 cells (HEK293).

Molecular Characterization
rhIL2RG Fc Chimera, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 53.5 kDa. The predicted N-terminus is Leu 23. DTT-reduced Protein migrates as 80-90 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhIL2RG Fc Chimera by the LAL method.

Purity
>98% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Kondo M., et al., 1993, Science 262:1874-1877.
(2) Russell S.M., et al., 1993, Science 262:1880-1883.
(3) Noguchi M., et al., 1993, Science 262:1877-1880.