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Human SIRP alpha / CD172a Protein, Fc Tag (HPLC-verified), 1mg  

Human SIRP alpha / CD172a Protein, Fc Tag (HPLC-verified), 1mg

Human SIRP alpha / CD172a Protein, Fc Tag (HPLC-verified) (Human SIRP alpha, Fc Tag (HPLC-verified)) Glu 31 - Arg 370 was produced in human 293 cells (HEK293)

Synonym
Recombinant Human Protein, SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1

More details

SIA-H5251-1K

Availability: within 7 days

1 980,00 €

Background
Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Source
Human SIRP alpha / CD172a Protein, Fc Tag (HPLC-verified) (Human SIRP alpha, Fc Tag (HPLC-verified)) Glu 31 - Arg 370 (Accession # NP_001035111) was produced in human 293 cells (HEK293).

Molecular Characterization
Human SIRP alpha, Fc Tag (HPLC-verified) is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 63.5 kDa. The predicted N-terminus is Glu 31. The reducing (R) protein migrates as 70-105 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human SIRP alpha, Fc Tag (HPLC-verified) by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Contact us for customized product form or formulation.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human SIRP alpha, Fc Tag (Catalog # SIA-H5251) at 2μ g/mL (100 μL/well) can bind Human CD47, His Tag (Catalog # CD7-H5227) with a linear range of 1.56-12.5 ng/mL.

References

(1) Timms J.F., et al., 1999, Curr. Biol. 9:927-930.
(2) Stofega M.R., et al., 2000, J. Biol. Chem. 275:28222-28229.
(3) Latour S., et al., 2001, J. Immunol. 167:2547-2554.