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Recombinant human CD47 Protein, His tag, 2x 500 µg  

Recombinant human CD47 Protein, His tag, 2x 500 µg

Recombinant Human CD47 Protein (Gln 19 - Pro 139), was produced in human 293 cells (HEK293), His tag

Synonym
Recombinant human protein, CD47, MER6, IAP, OA3

More details

CD7-H5227-1K

Availability: within 7 days

2 100,00 €

Background
Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

Source
Recombinant Human CD47 Protein (rhCD47) Gln 19 - Pro 139 (Accession # NP_942088) was produced in human 293 cells (HEK293).

Molecular Characterization
rhCD47, fused with 6×His tag at the C-terminus, has a calculated MW of 14.5 kDa. The predicted N-terminus is Gln 19. DTT-reduced Protein migrates as 35-43 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD47 by the LAL method.

Purity
>95% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human SIRP alpha, Fc Tag (Catalog # SIA-H5251) at 5 μg/mL (100 μL/well) can bind Human CD47, His Tag (Catalog # CD7-H5227) with a linear range of 2-13 ng/mL (QC tested).
Measured by its binding ability in a functional ELISA. Immobilized Anti-CD47 MAb, Human IgG4 (clone 5F9) at 2 μg/mL (100 μL/well) can bind Human CD47, His Tag (Catalog # CD7-H5227) with a linear range of 0.4-3 ng/mL (routinely tested).

References

(1) Lindberg F.P., et al., 1993, J. Cell Biol. 123:485-496.
(2) Latour S., et al., 2001, J. Immunol. 167:2547-2554.
(3) Piccio L., et al., 2005, Blood 105:2421-2427.