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Recombinant Human CD30 /TNFRSF8 Protein, His tag, 1mg  

Recombinant Human CD30 /TNFRSF8 Protein, His tag, 1mg

Recombinant Human CD30 /TNFRSF8 Protein (Phe 19 - Lys 379), produced in human 293 cells (HEK293), His tag

Synonym
recombinant human protein, TNFRSF8, CD30, D1S166E, Ki-1

More details

CD0-H5229-1K

Availability: within 7 days

1 500,00 €

Background
Human CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. TNFRSF-8 is expressed by activated, but not by resting, T and B cells. Also, CD30 is expressed on classical Hodgkin Lymphoma cells together with CD15. CD30 is the receptor for TNFSF8/CD30L. CD30 can interact with TRAF2 and TRAF5, and mediate the signal transduction that leads to the activation of NF-kappa-B. TNFRSF8 may play a role in the regulation of cellular growth and transformation of activated lymphoblasts. TNFRSF8 is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity.

Source
Recombinant Human CD30 /TNFRSF8 Protein (rhCD30) Phe 19 - Lys 379 (Accession # NP_001234.2) was produced in human 293 cells (HEK293).

Molecular Characterization
rhCD30, fused with 6×His tag at the C-terminus, has a calculated MW of 39.3 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 75-90 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD30 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. LIT-H5256) at 2 μg/mL (100 μL/well) can bind Biotinylated Human HVEM, Fc Tag (Cat. No. HV4-H82F1) with a linear range of 0.6-39 ng/mL (QC tested). Immobilized ActiveMax® Human TNF-beta (Cat. No. TNB-H5214) at 10 μg/mL (100 µl/well),can bind Biotinylated Human HVEM, Fc Tag (Cat. No. HV4-H82F1) with a linear range of 0.6-10 μg/mL (Routinely tested).

References

(1) Gorczyca W., et al., 2003, Int. J. Oncol. 22 (2): 319–24.
(2) Aizawa S., et al., 1997, J. Biol. Chem. 272 (4): 2042–5.
(3) Ansieau S., 1996, Proc. Natl. Acad. Sci. U.S.A. 93 (24): 14053–8.
(4) Lee S.Y., et al., 1996, J. Exp. Med. 183:669-674.