Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human CD200/OX-2 Protein, Fc Tag, 1mg  

Recombinant Human CD200/OX-2 Protein, Fc Tag, 1mg

Recombinant Human CD200 /OX-2 Protein, Gln 31 - Gly 232, was produced in human 293 cells (HEK293), Fc tag

Synonym
Recombinant Human Protein, CD200, MOX1, MOX2, MRC, OX-2, My033

More details

OX2-H5251-1K

Availability: within 7 days

1 440,00 €

Background
CD200 also known as OX-2 membrane glycoprotein (OX-2), is a type-1 membrane glycoprotein, which contains two immunoglobulin domains (1 Ig-like C2-type (immunoglobulin-like) domain and Ig-like V-type (immunoglobulin-like) domain), and thus belongs to the immunoglobulin superfamily. CD200 / OX-2 is widely expressed in multiple cell types. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. OX-2 / CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.

Source
Recombinant Human CD200 /OX-2 Protein, With C-Fc Tag (rhCD200 Fc Chimera) Gln 31 - Gly 232 (Accession # AAH22522) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 48.6 kDa. The protein migrates as 55-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD200 Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Please refer to product data sheet.

References

(1) McCaughan, G.W. et al., 1987, Immunogenetics 25:329-335.
(2) Chen, Z. et al., 2006, Mol Immunol. 43 (6): 579-587.
(3) Wright GJ., et al., 2003, J. Immunol. 171 (6): 3034–46.

The following products could also be interesting for you: