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Recombinant Cynomolgus / Rhesus macaque CTLA-4 Protein, Fc Tag, 1 mg  

Recombinant Cynomolgus / Rhesus macaque CTLA-4 Protein, Fc Tag, 1 mg

Recombinant Cynomolgus / Rhesus macaque CTLA4 / CD152 Protein, Ala 37 - Ser 160, was produced in human 293 cells (HEK293), Fc Tag

Synonym
Recombinant Cynomolgus Protein, CTLA4, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12

More details

CT4-C5256-1K

Availability: within 7 days

902,00 €

Background: CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

Source
Recombinant Cynomolgus / Rhesus macaque CTLA-4, Fc Tag (CT4-C5256) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Ser 160 (Accession # G7PL88).

Molecular Characterization
Cynomolgus CTLA4 /CD152 Fc Chimera, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 40 kDa. The predicted N-terminus is Ala 37. As a result of glycosylation, DTT-reduced Protein migrates as 45-52 kDa and non-reduced protein migrates as 90-105 kDa.

Endotoxin
Less than 1.0 EU per μg of the Cynomolgus CTLA4 /CD152 Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 3 months (-20°C to -70°C).

References

(1) Waterhouse P, et al., 1995, Science 270 (5238): 985–8.
(2) Magistrelli G, et al., 1999. Eur. J. Immunol. 29 (11): 3596–602.
(3) Rudd, CE. et al., 2009, Immunol. Rev. 229 (1): 12-26.

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