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Recombinant Human CD80 /  B7-1 Protein, His tag, 1 mg  

Recombinant Human CD80 / B7-1 Protein, His tag, 1 mg

Recombinant Human B7-1 / CD80 Protein (Val 35 - Asn 242), produced in human HEK293 cells, His tag

Synonym
recombinant human protein CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7

More details

B71-H5228-1K

Availability: within 7 days

1 700,00 €

Background
B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

Source
Human B7-1, His Tag (B71-H5228) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # P33681-1).

Molecular Characterization
rhCD80, fused with 6×His tag at the C-terminus, has a calculated MW of 24.7 kDa. The predicted N-terminus is Val 35. DTT-reduced Protein migrates as 45-75 kDa due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD80 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, His Tag (Cat. No. B71-H5228) at 20 μg/mL (100 µl/well),can bind Human CD28, Fc Tag (Cat. No. CD8-H5257) with a linear of 0.2-20 μg/mL (Routinely tested).
Measured by its binding ability in a functional ELISA. Immobilized Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) at 10 μg/mL (100 µl/well),can bind Human B7-1, His Tag (Cat. No. B71-H5228) with a linear range of 0.04-4 μg/mL (QC tested).

References

(1) Peach RJ., et al., 1995, J. Biol. Chem. (UNITED STATES) 270 (36): 21181–7.
(2) Stamper CC., et al., 2001, Nature (England) 410 (6828): 608–11.
(3) Bhatia S., et al., 2005, Proc. Natl. Acad. Sci. 102: 15569-15574.
(4) Khan N., et al., 2007, Int. Immunol. 19:477-486.

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