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Recombinant Cynomolgus / Rhesus macaque B7-1 / CD80 Protein (HPLC-verified), 2x500 µg  

Recombinant Cynomolgus / Rhesus macaque B7-1 / CD80 Protein (HPLC-verified), 2x500 µg

Recombinant Cynomolgus / Rhesus macaque CD80 / B7-1 Protein, Val 35 - Asn 242, produced in Human 293 cells (HEK293), His tag

Synonym
recombinant cynomolgus protein CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7

More details

CD0-C52H3-1K

Availability: within 7 days

1 760,00 €

Background
B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

Source
Cynomolgus / Rhesus macaque B7-1, His Tag (HPLC-verified) (CD0-C52H3) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # G7NXN7).
Predicted N-terminus: Val 35

Molecular Characterization
Cynomolgus CD80 / B7-1 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 25.8 kDa. The predicted N-terminus is Val 35. DTT-reduced Protein migrates as 43-55 kDa in SDS-PAGE due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Cynomolgus CD80 / B7-1 by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 3 months (-20°C to -70°C).

Bioactivity
n/a

References

(1) Peach RJ., et al., 1995, J. Biol. Chem. (UNITED STATES) 270 (36): 21181–7.
(2) Stamper CC., et al., 2001, Nature (England) 410 (6828): 608–11.
(3) Bhatia S., et al., 2005, Proc. Natl. Acad. Sci. 102: 15569-15574.
(4) Khan N., et al., 2007, Int. Immunol. 19:477-486.