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Human PD-L1 / B7-H1 Protein (HPLC-verified), 100µg  

Human PD-L1 / B7-H1 Protein (HPLC-verified), 100µg

Human PD-L1 / B7-H1 Protein (HPLC-verified) (Human PD-L1, His Tag (HPLC-verified)) Phe 19 - Arg 238 was produced in human 293 cells (HEK293)

Synonym
recombinant human protein PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

More details

PD1-H5229-100

Availability: within 7 days

348,00 €

Background
Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

Source
Human PD-L1 / B7-H1 Protein (HPLC-verified) (Human PD-L1, His Tag (HPLC-verified)) Phe 19 - Arg 238 (Accession # NP_054862.1 ) was produced in human 293 cells (HEK293)

Molecular Characterization
Human PD-L1, His Tag (HPLC-verified) is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 26 kDa. The predicted N-terminus is Phe 19. The reducing (R) protein migrates as 35-42 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human PD-L1, His Tag (HPLC-verified) by the LAL method.

Purity
>98% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization. 

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, His Tag (HPLC-verified) (Catalog # PD1- H5229) at 5 μg/mL ( 100 μl/well ) can bind Human PD-1, Fc Tag (Catalog # PD1-H5257) with a linear range of 0.05-1 μg/mL.

References

(1) Dong H., et al., 1999, Nat. Med. 5:1365-1369.
(2) Freeman G.J., et al., 2000, J. Exp. Med. 192:1027-1034.
(3) Nishimura H., et al., 2001, Trends in Immunology 22:265.