Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human PDL1 /CD274 Protein, Twin Strep Tag, 250µg  

Recombinant Human PDL1 /CD274 Protein, Twin Strep Tag, 250µg

Recombinant Human PDL1 /CD274 Protein, Phe 19 - Arg 238, produced in human 293 cells, Twin Strep Tag

Synonym
recombinant human protein PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

More details

PD1-H5282-250

Availability: within 7 days

968,00 €

Background
Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

Source
Recombinant Human PDL1 /CD274 Protein, With C-Twin Strep Tag (rh PDL1 /CD274,twin strep tag) Phe 19 - Arg 238 (Accession # NP_054862.1) was produced in human 293 cells (HEK293)

Molecular Characterization
rh PDL1 /CD274,twin strep tag is fused with Twin-Strep tag at the C-terminus, and has a calculated MW of 28.2 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 35-42 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh PDL1 /CD274,twin strep tag by the LAL method.

Purity
>95% as determined by SEC-HPLC and SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Bioactivity
Please refer to product data sheet.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 3 months (-20°C to -70°C).

References

(1) Dong H., et al., 1999, Nat. Med. 5:1365-1369.
(2) Freeman G.J., et al., 2000, J. Exp. Med. 192:1027-1034.
(3) Nishimura H., et al., 2001, Trends in Immunology 22:265.