Recombinant Cynomolgus / Rhesus macaque PD-L1 / B7-H1 Protein, His tag, 100µg

Background
Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

Source
Recombinant Cynomolgus / Rhesus macaque PD-L1, His Tag (PD1-C52H4) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # G7PSE7).

Molecular Characterization
Cynomolgus PDL1 /CD274 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 27.1 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 35-40 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Cynomolgus PDL1 /CD274 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Bioactivity
Immobilized Cynomolgus / Rhesus macaque PD-L1, His Tag (Cat. No. PD1-C52H4) at 10 μg/mL (100 μL/well) can bind Cynomolgus PD-1, Fc Tag (Cat. No. PD1-C5254) with a linear range of 0.6-10 μg/mL (QC tested).

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 3 months (-20°C to -70°C).

References