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Recombinant Human PD-L2 / B7-DC Protein, (HPLC-verified), Fc Tag, 1mg  

Recombinant Human PD-L2 / B7-DC Protein, (HPLC-verified), Fc Tag, 1mg

Recombinant human PD-L2 / B7-DC Protein, Leu 20 - Pro 219, produced in human 293 cells (HEK293), HPLC-verified, Fc Tag

Synonym
recombinant human protein PDL2, PD-L2, Butyrophilin B7-DC, CD273, PDCD1 ligand 2, PDCD1L2, PDCD1LG2

More details

PD2-H5251-1K

Availability: within 7 days

1 700,00 €

Background
Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

Source
Human PD-L2 / B7-DC Protein, Fc Tag (HPLC-verified) (Human PD-L2, Fc Tag (HPLC-verified) ) Leu 20 - Pro 219 was produced in human 293 cells (HEK293).

Molecular Characterization
Human PD-L2, Fc Tag (HPLC-verified) is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 49.2 kDa. The predicted N-terminus is Leu 20. The reducing (R) protein migrates as 60-70 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human PD-L2, Fc Tag (HPLC-verified) by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 3 months (-20°C to -70°C).

Bioactivity
Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 200 μg/mL (100 μL/well) can bind Human PD-L2, Fc Tag (Cat. No. PD2-H5251) with a linear range of 0.156-0.625 μg/mL (QC tested).
Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L2, Fc Tag (Cat. No. PD2-H5251) with an EC50 of 0.018 μg/mL (Routinely tested).
Flow Cytometry assay shows that recombinant human PD-L2 (Cat. No. PD2-H5251) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 1 μg/mL (Routinely tested).

References

(1) Zhang Z., et al., 2004, Protein Sci. 13:2819-2824.
(2) The MGC Project Team., et al., 2004, Genome Res. 14:2121-2127.
(3) Latchman Y., et al., 2001, Nat. Immunol. 2:261-268.