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Recombinant Human FCGR3A / CD16a (V176) Protein, His tag, 2x 500µg  

Recombinant Human FCGR3A / CD16a (V176) Protein, His tag, 2x 500µg

Recombinant Human FCGR3A / CD16a (V176) Protein, produced in human 293 cells, Gly 17 - Gln 208, His Tag (SPR & BLI & MALS verified)

Synonym
recombinant, human, protein, FCGR3A, CD16A, FCG3, FCGR3, IGFR3

More details

CD8-H52H4-1K

Availability: within 7 days

1 800,00 €

Background
CD16a antigen also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. In humans, CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two different highly homologous genes in a cell type-specific manner. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody­dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

Source
Recombinant Human FCGR3A / CD16a (V176) Protein, His Tag (CD8-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # P08637-1).
Predicted N-terminus: Gly 17

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 23.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human FCGR3A / CD16a (V176) Protein, His Tag by the LAL method.

Purity
>95% purity as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

BIAFFIN SERVICES:

Use BIAFFINS FcyR BINDING ASSAYS for detailed kinetic analysis of your antibodies by SPR.

Figure: Real-time kinetic analysis of FcγRIIIa binding to antibody using surface plasmon resonance.

Please contact our application specialists to obtain more information and an individual quote tailored to your specific needs.

References

(1) Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype
Congy-Jolivet, Bolzec, Ternant et al
Cancer Res (2008) 68 (4), 976-80

Showing 1-1 of 1 papers.
Please refer to product data sheet.

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