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Recombinant Human FcGR2A / CD32a (H167) Proteing, 100µg  

Recombinant Human FcGR2A / CD32a (H167) Proteing, 100µg

Recombinant Human FcGR2A / CD32a (H167) Protein, Ala 36 - Ile 218, produced in human 293 cells, His-Tag SPR verified

Synonym
Recombinant, human, protein CD32a,FCGR2A,CD32,FCG2 ,FCGR2A1,IGFR2

More details

CD1-H5223-100

Availability: within 7 days

336,00 €

Background
Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM­bearing receptors may also be coexpressed and co­engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

Source
Recombinant Human FcGR2A / CD32a (H167) Protein,also called Human FcGR2A / CD32a Protein (167 His), Ala 36 - Ile 218 (Accession # P12318-1), was produced in human 293 cells (HEK293).

Molecular Characterization
Human FcGR2A / CD32a (H167), His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 21.1 kDa. The predicted N-terminus is Ala 36. DTT-reduced Protein migrates as 29-32 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human FcGR2A / CD32a (H167), His Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Bioactivity 
Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Human LIGHT, Fc tag (Cat. No. LIT-H5265) with a linear range of 0.5-8 ng/mL (QC tested).

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Ravetch JV. et al., 2001, Annu Rev Immunol. 19: 275-83.
(2) Takai T. 2002, Nature Rev Immunol. 2: 580-92.
(3) Nagarajan S. et al., 2000, Blood. 3: 1069-77.(3) Güssow D., et al., 1987, J. Immunol. 139 (9): 3132–8.

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