Recombinant Mouse Fc gamma RI / CD64 Protein, His tag (MALS & BLI verified), 1mg

Background
Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.

Source
Recombinant Mouse CD64 protein, His Tag (CD4-M5227) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Pro 297 (Accession # P26151-1). Predicted N-terminus: Glu 25

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 32.3 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Bioactivity
Please refer to product data sheet.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Development of an Aptamer-Based Molecular Tool for Specifically Targeting Microglia via the CD64 Protein"
Zhu, Wu, Pan et al
Anal Chem (2023) 95 (6), 3238-3246
(2) "Neutrophil CD64 index for diagnosis of infectious disease in the pediatric ICU: a single-center prospective study"
Cao, Wang, Zhao et al
BMC Pediatr (2022) 22 (1), 718
(3) "Monocyte adaptations in patients with obesity during a 1.5 year lifestyle intervention"
van der Valk, Mulder, Kouwenhoven et al
Front Immunol (2022) 13, 1022361
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