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Human IFN-alpha / beta R2 Protein, Fc Tag,2x500 µg  

Human IFN-alpha / beta R2 Protein, Fc Tag,2x500 µg

Recombinant Human IFNAR2 /IFNABR Protein, Ile 27 - Lys 243, produced in human 293 cells (HEK293), Fc Tag

Synonym
recombinant, human, protein, IFNAR2,IFNARB,IFNABR,IFN-R-2,IFN-alpha/beta receptor 2

More details

IF2-H5255-1K

Availability: within 7 days

1 600,00 €

Background
Interferon alpha/beta receptor 2 (IFNAR2) is also known as IFN-alpha binding protein, IFN-alpha/beta receptor 2, Type I interferon receptor 2, IFNABR and IFNARB, which is a single-pass type I membrane protein and belongs to the type II cytokine receptor family. IFNAR2 can associate with IFNAR1 to form the type I interferon receptor. IFNAR2 is a receptor for interferons alpha and beta.IFNAR2 involves in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 of IFNAR2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 of IFNAR2 is a potent inhibitor of type I IFN receptor activity.Genetic variations in IFNAR2 influence susceptibility to hepatitis B virus (HBV) infection.

Source
Recombinant Human IFNAR2 /IFNABR Protein,C-Fc Tag (rh IFNAR2 /IFNABR Fc Chimera) Ile 27 - Lys 243 (Accession # NP_997467) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
rh IFNAR2 /IFNABR Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 51.4 kDa. The predicted N-terminus is Ile 27. DTT-reduced Protein migrates as 66-90 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh IFNAR2 /IFNABR Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Novick D., et al., 1994, Cell 77:391-400.
(2) Novick D., et al., 1995, J. Leukoc. Biol. 57:712-718.
(3) Li X., et al., 1997, Mol. Cell. Biol. 17:2048-2056.