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Recombinant Human CD36 / SR-B3 Protein, His Tag, 500µg  

Recombinant Human CD36 / SR-B3 Protein, His Tag, 500µg

Recombinant Human CD36 / SR-B3 Protein, Gly 30 - Asn 439, produced in human 293 cells (HEK293), His tag

Synonym
recombinant, human, protein, CD36,SCARB3,GP4,GPIV,PASIV,Platelet Glycoprotein 4,glycoprotein IV

More details

CD6-H5221-500

Availability: within 7 days

990,00 €

Background
CD36 (Cluster of Differentiation 36) is also known as platelet membrane glycoprotein IV (GPIV), fatty acid translocase (FAT), thrombospondin receptor, collagen receptor, and scavenger receptor class B, member 3 (SRB3), is a member of the class B scavenger receptor family of cell surface proteins. The human CD36 gene encodes a single chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.CD36 is found on platelets, erythrocytes, monocytes, differentiated adipocytes, mammary epithelial cells, spleen cells and some skin microdermal endothelial cells. CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta-amyloid fibrils (fAβ), collagens I and IV, and Plasmodium falciparum infected erythrocytes. CD36 is required for the anti-angiogenic effects of thrombospondin1 In the corneal neovascularization assay. On binding a ligand the protein and ligand are internalized. This internalization is independent of macropinocytosis and occurs by an actin dependent mechanism requiring the activation Src-family kinases, JNK and Rho-family GTPases. CD36 ligands have also been shown to promote sterile inflammation through assembly of a Toll-like receptor 4 and 6 heterodimer.

Source
Recombinant Human CD36 / SR-B3 Protein (Human CD36, His Tag) Gly 30 - Asn 439 (Accession # NP_001001547.1) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
Human CD36, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 47.5 kDa. The predicted N-terminus is Gly 30. The reducing (R) protein migrates as 60-90 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Husemann, J. et al., 2002, Glia 40:195.
(2) Armstrong, L and P. Bornstein, 2003, Matrix. Biol. 22:63.
(3) Collins RF, et al., 2009, J. Biol. Chem. 284 (44): 30288–97.
(4) Stewart CR, et al., 2010, Nat. Immunol. 11 (2): 155–61.