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Recombinant Mouse IL-13 R alpha 1 Protein, Fc Tag, 2 x 500 µg  

Recombinant Mouse IL-13 R alpha 1 Protein, Fc Tag, 2 x 500 µg

Recombinant Mouse IL13RA1 Protein, Ala 26 - Thr 340, produced in human 293 cells (HEK293), Fc Tag

Synonym
recombinant, mouse, protein, IL13RA1, CD213A1, IL-13Ra

More details

IL1-M5258-1K

Availability: within 7 days

880,00 €

Background
Interleukin 13 receptor, alpha 1 is also known as IL13RA1, NR4 and CD213A1 (cluster of differentiation 213A1), The IL13 Rα1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region and a 59 aa residue cytoplasmic tail. Human and mouseIL13Rα1 share 76% aa sequence identity. IL13RA1 is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.

Source
Recombinant Mouse IL13RA1 Protein,C-Fc Tag (Mouse IL13RA1 Fc Chimrea) Ala 26 - Thr 340 (Accession # NP_598751) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
Mouse IL13RA1 Fc Chimrea is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 62.7 kDa. The predicted N-terminus is Ala 26. DTT-reduced Protein migrates as 67-100 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Mouse IL13RA1 Fc Chimrea by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).


References

(1) Caput D. et al., 1996, J. Biol. Chem, 271: 16921.
(2) Donaldson DD., et al., 1998, J. Immunol, 161: 2317.
(3) Hilton DJ, et al., 1996, Proc. Natl. Acad. Sci. USA, 93:497.