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Recombinant Human IL-2 R alpha / CD25 Protein, His tag, 1mg  

Recombinant Human IL-2 R alpha / CD25 Protein, His tag, 1mg

Recombinant Human IL2RA /CD25 Protein, Glu 22 - Cys 213, expressed in human 293 cells (HEK293), His tag

Synonym: recombinant, human, protein, IL2RA, CD25, p55,IL2-RA, IL-2-RA

More details

ILA-H52H9-1K

Availability: within 7 days

1 800,00 €

Background
Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.

Source
Recombinant Human IL-2 R alpha, His Tag (ILA-H52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408).
Predicted N-terminus: Glu 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.6 kDa. The protein migrates as 34-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh IL2RA /CD25 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity-ELISA
Immobilized Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) with a linear range of 1-39 ng/mL (QC tested).

Bioactivity-SPR
Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 29.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Loaded Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) on HIS1K Biosensor, can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 18 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-Cell-based assay
Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) inhibits the IL-2 dependent proliferation of Mo7e cells. The EC50 for this effect is 0.35-0.77 µg/mL (Routinely tested).

References

(1) Leonard W.J., et al., 1985, Science 230:633-639.
(2) Miedel M.C., et al., 1988, Biochem. Biophys. Res. Commun. 154:372-379.
(3) Wang X., et al., 2005, Science 310:1159-1163.