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Recombinant Human IL-18 R1 / CD218a Protein, Fc Tag, 200µg  

Recombinant Human IL-18 R1 / CD218a Protein, Fc Tag, 200µg

Recombinant Human IL18R1 /CD218a Protein, Ala 19 - Arg 329, produced in human 293 cells (HEK293), Fc Tag

Synonym
recombinant, human, protein, IL18R1, IL1RRP, CD218a, IL18Ra

More details

IL1-H5259-200

Availability: within 7 days

348,00 €

Background
Interleukin-18 receptor 1 (IL18R1) is also known as CD218 antigen-like family member A (CD218a), IL1 receptor-related protein (IL-1Rrp or IL1R-rp), CDw218a, IL18Ra, IL1RRP, which belongs to the interleukin-1 receptor family. IL18R1 contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL18R1 is receptor for interleukin 18 (IL-18). IL18R1 binds to the agonist leads to the activation of NF-kappa-B, but does not bind IL1A/interleukin-1 alpha or IL1B/interleukin-1 beta.

Source
Recombinant Human IL18R1 /CD218a Protein, With C-Fc Tag (rh IL18R1 /CD218a Fc Chimera) Ala 19 - Arg 329 (Accession # AAH69575) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
rh IL18R1 /CD218a Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 62.4 kDa. The predicted N-terminus is Ala 19. DTT-reduced Protein migrates as 90-110 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh IL18R1 /CD218a Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Parnet P., et al., 1996, J. Biol. Chem. 271:3967-3970.
(2) Torigoe K., et al., 1997, J. Biol. Chem. 272:25737-25742.