Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human TRAIL R2 / DR5 / TNFRSF10B Protein,  His tag, 1 mg  

Recombinant Human TRAIL R2 / DR5 / TNFRSF10B Protein, His tag, 1 mg

Recombinant Human TRAIL R2 / DR5 / TNFRSF10B Protein, Ile 56 - Glu 182, produced in human 293 cells (HEK293), His Tag

Synonym
recombinant, human, protein, TNFRSF10B,TRAILR2,TRAIL-R2,CD262, DR5

More details

TR2-H5229-1K

Availability: within 7 days

1 600,00 €

Background
Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) is also known as TNF-related apoptosis-inducing ligand receptor 2 (TRAILR2), Death receptor 5 (DR5), CD262, KILLER, is a member of the TNF-receptor superfamily, and contains an intracellular death domain. TNFRSF10B / DR-5 is widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines. TRAILR2 / CD262 / DR5 is the receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD (a death domain containing adaptor protein) of TRAIL-R2 / TNFRSF10B recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. CD262 / DR5 Promotes the activation of NF-kappa-B. DR5 is essential for ER stress-induced apoptosis and is regulated by p53/TP53.

Source
Human TRAIL R2, His Tag (TR2-H5229) is expressed from human 293 cells (HEK293). It contains AA Ile 56 - Glu 182 (Accession # NP_003833).
Predicted N-terminus: Ile 56

Molecular Characterization
Human TRAIL R2, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 15.1 kDa. The predicted N-terminus is Ile 56. The reducing (R) protein migrates as 15-20 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity-ELISA
Immobilized Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) at 5 μg/mL (100 μL/well) can bind Human TRAIL R2, His Tag (Cat. No. TR2-H5229) with a linear range of 10-78 ng/mL (QC tested).

References

(1) Yamaguchi H., et al., 2004, J. Biol. Chem. 279:45495-45502.
(2) Gajate Consuelo, et al., 2005, J. Biol. Chem. (United States) 280 (12): 11641–7.
(3) Chaudhary, P M., et al., 1997, Immunity (UNITED STATES) 7 (6): 821–30.