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Recombinant Human TRAIL R2 / DR5 / TNFRSF10B Protein, Fc Tag, 1 mg  

Recombinant Human TRAIL R2 / DR5 / TNFRSF10B Protein, Fc Tag, 1 mg

Recombinant Human TRAIL R2 / DR5 / TNFRSF10B Protein, Ile 56 - Glu 182, produced in human 293 cells (HEK293), Fc Tag

Synonym
recombinant, human, protein, TNFRSF10B,TRAILR2,TRAIL-R2,CD262,DR5

More details

TR2-H5255-1K

Availability: within 7 days

1 600,00 €

Background
Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) is also known as TNF-related apoptosis-inducing ligand receptor 2 (TRAILR2), Death receptor 5 (DR5), CD262, KILLER, is a member of the TNF-receptor superfamily, and contains an intracellular death domain. TNFRSF10B / DR-5 is widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines. TRAILR2 / CD262 / DR5 is the receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD (a death domain containing adaptor protein) of TRAIL-R2 / TNFRSF10B recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. CD262 / DR5 Promotes the activation of NF-kappa-B. DR5 is essential for ER stress-induced apoptosis and is regulated by p53/TP53.

Source
Recombinant Human TRAIL R2, Fc Tag (TR2-H5255) is expressed from human 293 cells (HEK293). It contains AA Ile 56 - Glu 182 (Accession # NP_003833).
Predicted N-terminus: Ile 56.

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 40.5 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human TRAIL R2, Fc Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity
ELISA
Immobilized Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) at 5 μg/mL (100 μL/well) can bind Human TRAIL R2, Fc Tag (Cat. No. TR2-H5255) with a linear range of 0.6-20 ng/mL (QC tested).

BLI
Loaded Human TRAIL R2, Fc Tag (Cat. No. TR2-H5255) on AHC Biosensor, can bind Human TRAIL, His Tag with an affinity constant of 0.146 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

References

(1) Yamaguchi H., et al., 2004, J. Biol. Chem. 279:45495-45502.
(2) Gajate Consuelo, et al., 2005, J. Biol. Chem. (United States) 280 (12): 11641–7.
(3) Chaudhary, P M., et al., 1997, Immunity (UNITED STATES) 7 (6): 821–30.