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Recombinant Human Siglec-3 / CD33 Protein, Fc Tag, 1 mg  

Recombinant Human Siglec-3 / CD33 Protein, Fc Tag, 1 mg

Recombinant Human Siglec-3 / CD33 Protein, Asp 18 - His 259, produced in human 293 cells (HEK293), Fc Tag

Synonym
recombinant, human, protein, CD33,SIGLEC3,gp67

More details

CD3-H5257-1K

Availability: within 7 days

1 600,00 €

Background
Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.

Source
Recombinant Human Siglec-3 / CD33 Protein, Fc Tag (Human Siglec-3, Fc Tag) Asp 18 - His 259 (Accession # AAH28152.1) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
Human Siglec-3, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 52.9 kDa. The predicted N-terminus is Asp 18. DTT-reduced Protein migrates as 64-85 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human Siglec-3, Fc Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Bioactivity
Please refer to product data sheet.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Garnache-Ottou F., et al., 2005, Blood 105 (3): 1256–64.
(2) Hernández-Caselles T, et al., 2006, J. Leukoc. Biol. 79 (1): 46–58.
(3) Walter RB, et al., 2007, Blood 109 (10): 4168–70.
(4) Ulyanova, T. et al., 1999, Eur. J. Immunol. 29:3440.
(5) Crocker, P.R. and A. Varki, 2001, Immunology 103:137.