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Recombinant human CD38 Protein, His tag, 2 x 500µg  

Recombinant human CD38 Protein, His tag, 2 x 500µg

Recombinant Human CD38 Protein (Val 43 - Ile 300), produced in human 293 cells (HEK293), His tag (MALS verified)

Synonym
recombinant, human, protein, CD38,T10,cADPr hydrolase 1

More details

CD8-H5224-1K

Availability: within 7 days

2 178,00 €

Background
CD antigen CD38 is also known as ADP-ribosyl cyclase 1, which belongs to the ADP-ribosyl cyclase family. CD38 is expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. These reaction products are essential for the regulation of intracellular Ca2+. The loss of CD38 function is associated with impaired immune responses, metabolic disturbances, and behavioral modifications. The CD38 protein is a marker of cell activation. It has been connected to HIV infection, leukemias, myelomas, solid tumors, type II diabetes mellitus and bone metabolism. CD38 has been used as a prognostic marker in leukemia.

Source
Recombinant human CD38 Protein, His Tag (CD8-H5224) is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300 (Accession # NP_001766).
Predicted N-terminus: Val 43

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 30.7 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM MES, pH 6.5 with 100 mM NaCl. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity
Please refer to product data sheet.

References

(1) Orciani M., et al., 2008, J. Cell. Biochem. 105 (3): 905–12.
(2) Nata K., et al., 1997, Gene 186 (2): 285–92.
(3) Malavasi F., et al., 2008, Physiol. Rev. 88 (3): 841–86.