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Recombinant Human IL-7 R alpha / CD127 Protein, Fc Tag, 2 x 500µg  

Recombinant Human IL-7 R alpha / CD127 Protein, Fc Tag, 2 x 500µg

Recombinant Human IL-7 R alpha / CD127 Protein (Glu 21 - Gly 236), produced in human 293 cells (HEK293), Fc Tag

Synonym
recombinant, human, protein, IL7Ra,CD127

More details

ILA-H5258-1K

Availability: within 7 days

1 600,00 €

Background
Interleukin-7 receptor subunit alpha (IL7Ra) is also known as CD antigen CD127, which belongs to the type I cytokine receptor family and type 4 subfamily. IL7Ra /CD127 contains 1 fibronectin type-III domain. IL7Ra /CD127 is expressed on various cell types, including naive and memory T cells and many others. IL7Ra /CD127 is receptor for interleukin-7 and also acts as a receptor for thymic stromal lymphopoietin (TSLP). The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.

Source
Recombinant Human IL-7 R alpha / CD127 Protein, Fc Tag (Human IL-7 R alpha, Fc Tag) Glu 21 - Gly 236 (Accession # NP_002176) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
Human IL-7 R alpha, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 51 kDa. The predicted N-terminus is Glu 21. The reducing (R) protein migrates as 66-90 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

Bioactivity-ELISA
Immobilized ActiveMax® Human IL-7, Tag Free (Cat. No. IL7-H4219) at 2 μg/mL (100 μL/well) can bind Human IL-7 R alpha, Fc Tag (Cat. No. ILA-H5258) with a linear range of 1-16 ng/mL (QC tested).

Bioactivity-SPR
Captured Human IL-7 R alpha, Fc Tag (Cat. No. ILA-H5258) on CM5 chip via anti-human IgG Fc antibodies surface can bind ActiveMax® Human IL-7, Tag Free (Cat. No. IL7-H4219) with an affinity constant of 23.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

References

(1) Noguchi M., et al., 1994, Science 262 (5141): 1877–80.
(2) Kroemer RT., et al., 1997, Protein Eng. 9 (12): 1135–42.
(3) McElroy C.A., et al., 2009, Structure 17:54-65.