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Recombinant Human Cadherin-6 / KCAD / CDH6 Protein, His tag, 2 x 500µg  

Recombinant Human Cadherin-6 / KCAD / CDH6 Protein, His tag, 2 x 500µg

Recombinant Human CDH6 / KCAD / Cadherin-6 Protein (Thr 19 - Ala 615), produced in human 293 cells (HEK293), His tag

Synonym
Recombinant human protein, CDH6, CAD6, KCAD, K-cadherin, Cadherin-6

More details

CA6-H5229-1K

Availability: within 7 days

1 500,00 €

Background
Cadherin-6 (CDH6) also known as Kidney cadherin (K-cadherin or KCAD), is a a type II classical cadherin from the cadherin superfamily. Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH6 / KCAD contains five cadherin domains. CDH6 is highly expressed in brain, cerebellum, and kidney. Lung, pancreas, and gastric mucosa show a weak expression and also expressed in certain liver and kidney carcinomas.

Source
Recombinant Human CDH6 /Cadherin-6 Protein (rhCDH6 /Cadherin-6) Thr 19 - Ala 615 (Accession # AAH00019) was produced in human 293 cells (HEK293) at ACRObiosystems.

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The Human Cadherin-6 will be further processed into mature form (Ser 54-Ala 615). The protein has a calculated MW of 67.2 kDa (pro-form) and 63.4 kDa (mature-form). The protein migrates as 86-96 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCDH6 /Cadherin-6 by the LAL method.

Purity
>85% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Suzuki S., et al., 1991, Cell Regul. 2:261-270.
(2) MacCalman CD., et al., 1998, Am. J. Reprod. Immunol. 39 (2): 96–107.