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Recombinant Human CD98 Protein, His tag, 2 x 500µg  

Recombinant Human CD98 Protein, His tag, 2 x 500µg

Recombinant Human CD98 Protein (Arg 206 - Ala 630), produced in human 293 cells (HEK293), His tag

Synonym
Recombinant human protein, SLC3A2,CD98,4F2,4F2HC,4T2HC,CD98HC

More details

CD8-H5247-1K

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1 700,00 €

Background
CD antigen CD98 is also known as 4F2 cell-surface antigen heavy chain (4F2hc), 4F2 heavy chain antigen, Solute carrier
family 3 member 2 (SLC3A2), Lymphocyte activation antigen 4F2 large subunit, is a single-pass type I I membrane protein
which belongs to the SLC3A transporter family.. SLC3A2 / CD98 is expressed ubiquitously in all tissues tested with highest
levels detected in kidney, placenta and testis and weakest level in thymus. SLC3A2 / CD98 is required for the function of
light chain amino-acid transporters and also involved in sodium-independent, high-affinity transport of large neutral amino
acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. CD98 involved in guiding and targeting of LAT1 and
LAT2 to the plasma membrane. When associated with SLC7A5 or SLC7A8, CD98 involved in the cellular activity of small
molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane.
Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering
intracellular signals.

Source
Recombinant human CD98 Protein (Human CD98, His Tag) Arg 206 - Ala 630  (Accession # AAH01061) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 47.7 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human CD98, His Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Yanagida O., et al., 2001, Biochim. Biophys. Acta 1514:291-302.
(2) Mastroberardino L., et al., 1998, Nature 395:288-291.
(3) Okamoto Y., et al., 2002, Am. J. Physiol. 282:C196-C204.
(4) Li S., et al., 2005, J. Biol. Chem. 280:20102-20110.