Recombinant Human EGFR Protein, Twin Strep Tag, 25 µg

Background
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

Source
Recombinant Human EGF R, Strep Tag (EGR-H5285) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # NP_005219.2).
Predicted N-terminus: Leu 25

Molecular Characterization
rh EGFR /ErbB1,twin strep tag is fused with a Twin-Strep tag at the C-terminus, and has a calculated MW of 76.1 kDa. The predicted N-terminus is Leu 25. DTT-reduced Protein migrates as 110-115 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh EGFR /ErbB1,twin strep tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Herbst RS, 2004, Int. J. Radiat. Oncol. Biol. Phys. 59 (2 Suppl): 21–6.
(2) Zhang H, et al.,2007, Journal of Clinical Investigation 117(8):2051-2058.