Recombinant Human EGFR Protein, Twin Strep Tag, 25 µg

Background
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

Source
Recombinant Human EGF R, Strep Tag (EGR-H5285) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # NP_005219.2).
Predicted N-terminus: Leu 25

Molecular Characterization
This protein carries a twin strep tag at the C-terminus.
The protein has a calculated MW of 71.6 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh EGFR /ErbB1,twin strep tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Herbst RS, 2004, Int. J. Radiat. Oncol. Biol. Phys. 59 (2 Suppl): 21–6.
(2) Zhang H, et al.,2007, Journal of Clinical Investigation 117(8):2051-2058.