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Recombinant Human CD3 epsilon Protein, Twin Strep tag, 25 µg  

Recombinant Human CD3 epsilon Protein, Twin Strep tag, 25 µg

Recombinant Human CD3E /CD3 Epsilon Protein (Asp 23 - Asp 126 ), produced in human 293 cells (HEK293), C-Twin Strep tag

Synonym
Recombinant human protein, FLJ18683, T3E, TCRE, CD3E, CD3-epsilon

More details

CDE-H5286-25

Availability: within 7 days

312,00 €

Background
CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

Source
Recombinant Human CD3E /CD3 Epsilon Protein, With C-Twin Strep Tag (rh CD3E,twin strep tag) Asp 23 - Asp 126 (Accession # NP_000724.1) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
This protein carries a twin strep tag at the C-terminus.
The protein has a calculated MW of 14.8 kDa. The protein migrates as 16-20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh CD3E,twin strep tag by the LAL method.

Purity
>80% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Anti Human CD3 at 2 μg/mL (100 μL/well) can bind Human CD3E, Strep Tag (Cat. No. CDE-H5286) with a linear range of 0.2-3 ng/mL (Routinely tested).

SDS-Page
Human CD3E, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 80%.

References

(1) Gold DP, et al., 1986, Nature 321 (6068): 431–4.
(2) Clevers HC, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (21): 8156–60.
(3) de Saint Basile G, et al., 2004, J. Clin. Invest. 114 (10): 1512–7.
(4) Wong S, et al., 1991, Clin. Exp. Immunol. 83 (1): 69–73.

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