Recombinant Mouse gp130 / CD130 / IL-6 R beta Protein, Fc Tag, 100 µg

Background
Interleukin-6 receptor subunit beta (IL6ST) is also known as IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, Interleukin-6 signal transducer, Membrane glycoprotein 130 (gp130), CD130, Oncostatin-M receptor subunit alpha and Il6st,which is single-pass type I membrane protein. IL6ST /gp130 /CD130 can be found in tissues such as brain, heart, thymus, spleen, kidney, lung and liver and found in all the cell lines tested except BaF-B03.The expression of IL-6ST /gp130 is not restricted to IL6-responsive cells. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. IL6ST /CD130 can bind to IL6 /IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduce the signal. IL6ST /GP130 does not bind IL6 and may have a role in embryonic development.

Source
Recombinant Mouse IL6ST /GP130 /CD130 Protein,Fc Tag (ILT-M5252) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Glu 617 (Accession # NP_034690).
Predicted N-terminus: Gln 23

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 93.3 kDa. The protein migrates as 120-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95 % as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "ID factors regulate the ability of Müller glia to become proliferating neurogenic progenitor-like cells"
Taylor, Patel, Hawthorn et al
Glia (2024)
(2) "Genetic evidence for the causal effects of C-reactive protein on self-reported habitual sleep duration"
Iakunchykova, Pan, Amlien et al
Brain Behav Immun Health (2024) 37, 100754
(3) "Immunotherapeutic IL-6R and targeting the MCT-1/IL-6/CXCL7/PD-L1 circuit prevent relapse and metastasis of triple-negative breast cancer"
Haq, Yang, Jin et al
Theranostics (2024) 14 (5), 2167-2189
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