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Recombinant Mouse gp130 / CD130 / IL-6 R beta Protein, Fc Tag, 2x500 µg  

Recombinant Mouse gp130 / CD130 / IL-6 R beta Protein, Fc Tag, 2x500 µg

Recombinant Mouse IL6ST /GP130 /CD130 Protein (Gln 23 - Glu 617), produced in human 293 cells (HEK293), Fc tag

Synonym
Recombinant mouse protein, IL6ST,gp130,CD130,IL-6RB,IL-6R-beta,CDw130

More details

ILT-M5252-1K

Availability: within 7 days

1 800,00 €

Background
Interleukin-6 receptor subunit beta (IL6ST) is also known as IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, Interleukin-6 signal transducer, Membrane glycoprotein 130 (gp130), CD130, Oncostatin-M receptor subunit alpha and Il6st,which is single-pass type I membrane protein. IL6ST /gp130 /CD130 can be found in tissues such as brain, heart, thymus, spleen, kidney, lung and liver and found in all the cell lines tested except BaF-B03.The expression of IL-6ST /gp130 is not restricted to IL6-responsive cells. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. IL6ST /CD130 can bind to IL6 /IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduce the signal. IL6ST /GP130 does not bind IL6 and may have a role in embryonic development.

Source
Recombinant Mouse IL6ST /GP130 /CD130 Protein,C-Fc Tag (Mouse IL6ST /GP130 /CD130 Fc Chimera) Gln 23 - Glu 617 (Accession # NP_034690) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
Mouse IL6ST /GP130 /CD130 Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 93.3 kDa. The predicted N-terminus is Gln 23. DTT-reduced Protein migrates as 120-130 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Mouse IL6ST /GP130 /CD130 Fc Chimera by the LAL method.

Purity
>90 % as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
 

References

(1) Saito M., et al., 1992, J. Immunol. 148:4066-4071.
(2) Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.
(3) Tanaka M., et al., 1999, Blood 93:804-815.