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Recombinant Human Angiopoietin-like 4 / ANGPTL4 (166-406) Protein, His tag, 2 x 500µg  

Recombinant Human Angiopoietin-like 4 / ANGPTL4 (166-406) Protein, His tag, 2 x 500µg

Recombinant Human Angiopoietin-like 4 / ANGPTL4 (Pro 166 - Ser 406) Protein, produced in human 293 cells (HEK293), His tag

Synonym
Recombinant Human protein, ANGPTL4,ANGPTL2,ARP4,FIAF,HFARP,NL2,PGAR,pp1158,Angiopoietin like 4

More details

AN4-H5243-1K

Availability: within 7 days

1 440,00 €

Background
Angiopoietin-related protein 4 (ANGPTL4) is also known as Hepatic fibrinogen / angiopoietin - related protein (HFARP), ARP4, PGAR. ANGPTL4 contains one fibrinogen C-terminal domain. ANGPTL4 is a homooligomer , and the homooligomer undergoes proteolytic processing to release its carboxyl fibrinogen-like domain, which circulates as a monomer. The homooligomer unprocessed form is able to interact with the extracellular matrix. ANGPTL4 may act as a regulator of angiogenesis and modulate tumorigenesis. ANGPTL4 inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. ANGPTL4 may exert a protective function on endothelial cells through an endocrine action.

Source
Human Angiopoietin-like 4 (166-406), His Tag (AN4-H5243) is expressed from human 293 cells (HEK293). It contains AA Pro 166 - Ser 406 (Accession # NP_647475).
Predicted N-terminus: His

Molecular Characterization
Human Angiopoietin-like 4 (166-406), His Tag is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 27.9 kDa. The predicted N-terminus is His. DTT-reduced Protein migrates as 33-36 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human Angiopoietin-like 4 (166-406), His Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM MOPS, 100 mM NaCl, pH7.2 with 0.1% CHAPS. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Yoon J.C., et al., 2000, Mol. Cell. Biol. 20:5343-5349.
(2) Ito Y., et al., 2003, Cancer Res. 63:6651-6657.
(3) Zhu H., et al., 2002, Zhonghua Zhong Liu Za Zhi 24:123-125.