Recombinant Human Frizzled-7 / FZD7 Protein, Fc Tag, 4 x 250µg

Background
Frizzled-7 (FZD7) is also known as FzE3, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD7 contains one FZ (frizzled) domain. FZD7 is receptor for Wnt proteins. FZD7 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. FZD7 interacts with MAGI3 and DVL1.

Source
Recombinant Human FZD7 /Frizzled-7 Protein, with C-Fc Tag (rh FZD7 / Frizzled-7 Fc Chimera) Gln 33 - Leu 185 (Accession # AAH15915) was produced in human 293 cells (HEK293) at ACRObiosystems.

Molecular Characterization
rh FZD7 / Frizzled-7 Fc Chimera, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 43.3 kDa. The predicted N-terminus is Gln 33. DTT-reduced Protein migrates as 50-55 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh FZD7 / Frizzled-7 Fc Chimera by the LAL method.

Purity
>92% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Sagara N., et al., 1998, Biochem. Biophys. Res. Commun. 252:117-122.