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Recombinant Human IL-1RL1 / ST2 Protein, His tag, 2 x 500 µg  

Recombinant Human IL-1RL1 / ST2 Protein, His tag, 2 x 500 µg

Recombinant Human IL-1RL1 / ST2 Protein (Lys 19 - Phe 328), was produced in human 293 cells (HEK293) at ACROBiosystems, His tag

Synonym
recombinant, human, protein, IL1RL1,DER4,FIT-1,IL33R,ST2,ST2L,ST2V,T1

More details

IL1-H5229-1K

Availability: within 7 days

1 200,00 €

Background
Interleukin 1 receptor-like 1 (IL1RL1) is also known as ST2, DER4, FIT-1, IL33R, ST2L, ST2V, which belongs to the interleukin-1 receptor family. IL1RL1 contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL1RL1 is receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL1RL1 possibly involved in helper T-cell function. IL1RL1 can interact with MYD88, IRAK1, IRAK4, and TRAF6.

Source
Human IL-1RL1, His Tag (IL1-H5229) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - Phe 328 (Accession # Q01638-2).
Predicted N-terminus: Lys 19

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.9 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity
Immobilized human IL33 at 5 μg/mL (100 μL/well) can bind Human IL-1RL1, His Tag (Cat. No. IL1-H5229) with a linear range of 0.125-0.5 μg/mL (QC tested).

References

(1) Tago K., et al., 2001, Biochem. Biophys. Res. Commun. 285:1377-1383.
(2) Schmitz J., et al., 2005, Immunity 23:479-490.