Recombinant Human Siglec-5 / CD170 Protein, Fc Tag, 2 x 500 µg

Background
Sialic acid-binding Ig-like lectin 5 (SIGLEC5) is also known as CD33 antigen-like 2 (CD33L2), Obesity-binding protein 2 (OB-BP2), CD antigen CD170, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. SIGLEC5 / CD170 is expressed by monocytic / myeloid lineage cells. SIGLEC-5 / CD33-L2 binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Source
Recombinant Human SIGLEC5 /OB-BP2 /CD170 Protein, With C-Fc Tag (rh SIGLEC5 / OB-BP2 / CD170 Fc Chimera) Glu 17 - Thr 434 (Accession # AAH29896) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 73.0 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh SIGLEC5 / OB-BP2 / CD170 Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Cornish A.L., et al., 1998, Blood 92:2123-2132.
(2) Patel N., et al., 1999, J. Biol. Chem. 274:22729-22738.
(3) Zhuravleva M.A., et al., 2008, J. Mol. Biol. 375:437-447.