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Recombinant Human HSP90AA1 / HSP86 Protein, Tag Free, 100 µg  

Recombinant Human HSP90AA1 / HSP86 Protein, Tag Free, 100 µg

Recombinant Human HSP90AA1 / HSP 90-alpha Protein (Glu 535 - Asp 732 ), produced in E.coli cells, tag free

Synonym
Recombinant Human protein, HSP90AA1, HSP90A, HSP 86, HSP86, HSPC1, HSPCA, EL52, HSP89A, HSP90N, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2

More details

HS1-H511y-100

Availability: within 7 days

300,00 €

Background
Heat shock protein HSP 90-alpha (HSP90AA1 or HSP90A) is also known as Heat shock 86 kDa (HSP 86 or HSP86), Renal carcinoma antigen NY-REN-38, HSPC1, HSPCA, EL52, HSP89A, HSP90N, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2, which belongs to the heat shock protein 90 family. HSP90AA1 undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. HSP90AA1 interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

Source
Recombinant Human HSP90AA1 / HSP 90-alpha Protein (rh HSP90AA1 / HSP 90-alpha) Glu 535 - Asp 732 (Accession # NP_005339) was produced in E.coli cells at ACRObiosystems.

Molecular Characterization
rh HSP90AA1 / HSP 90-alpha, contains no "tag", has a calculated MW of 22.5 kDa. The predicted N-terminus is Gly. DTT-reduced Protein migrates as 24 kDa.

Endotoxin
Less than 1.0 EU per μg of the rh HSP90AA1 / HSP 90-alpha by the LAL method.

Purity
>82% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, pH7.0, with 150 mM NaCl. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Martinez-Ruiz A., et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102:8525-8530.
(2) Forsythe H.L., et al., 2001, J. Biol. Chem. 276:15571-15574.
(3) Yang J., et al., 2005, EMBO J. 24:1-10.