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Recombinant Human ALCAM Protein, His tag, 4 x 250µg  

Recombinant Human ALCAM Protein, His tag, 4 x 250µg

Recombinant Human ALCAM Protein (Trp 28 - Ala 526), produced in human 293 cells (HEK293), His tag

Synonym
Recombinant Human protein, CD166,ALCAM,CD6L,KG-CAM,MEMD

More details

CD6-H5228-1K

Availability: within 7 days

1 012,00 €

Background
CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins, which is also known as Activated leukocyte cell adhesion molecule (ALCAM) in human, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. CD166 is a cell adhesion molecule that binds to CD6. CD166 involved in neurite extension by neurons via heterophilic and homophilic interactions.

Source
Human ALCAM, His Tag (CD6-H5228) is expressed from human 293 cells (HEK293). It contains AA Trp 28 - Ala 526 (Accession # Q13740-1).
Predicted N-terminus: Trp 28

Molecular Characterization
Human ALCAM, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 56.7 kDa. The predicted N-terminus is Trp 28. The reducing (R) protein migrates as 65-80 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Degen W.G., et al., 1998, Am. J. Pathol. 152:805-813.
(2) Skonier J.E., et al., 1996, Biochemistry 35:12287-12291.
(3) Bajorath J., et al., 1995, Protein Sci. 4:1644-1647.