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Recombinant Human CD3 epsilon Protein, His Tag & Fc Tag, 1 mg  

Recombinant Human CD3 epsilon Protein, His Tag & Fc Tag, 1 mg

Recombinant Human CD3E /CD3 Epsilon Protein (Asp 23 - Asp 126), produced in human 293 cells (HEK293), His tag, Fc tag

Synonym
Recombinant human protein, FLJ18683, T3E, TCRE, CD3E, CD3-epsilon

More details

CDE-H5256-1K

Availability: within 7 days

1 870,00 €

Background
CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

Source
Human CD3 epsilon, Fc,His Tag (CDE-H5256) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # NP_000724.1).
Predicted N-terminus: Asp 23

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 39.2 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh CD3E Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity
Immobilized Anti Human CD3 MAb, mouse IgG1 (Clone # SP34-2) at 2 μg/mL (100 μL/well) can bind Human CD3 epsilon, Fc,His Tag (Cat. No. CDE-H5256) with a linear range of 0.1-3 ng/mL (Routinely tested).

References

(1) Gold DP, et al., 1986, Nature 321 (6068): 431–4.
(2) Clevers HC, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (21): 8156–60.
(3) de Saint Basile G, et al., 2004, J. Clin. Invest. 114 (10): 1512–7.
(4) Wong S, et al., 1991, Clin. Exp. Immunol. 83 (1): 69–73.