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Recombinant Human CD3 epsilon Protein, His Tag & Fc Tag, 1 mg  

Recombinant Human CD3 epsilon Protein, His Tag & Fc Tag, 1 mg

Recombinant Human CD3E /CD3 Epsilon Protein (Asp 23 - Asp 126), produced in human 293 cells (HEK293), His tag, Fc tag

Synonym
Recombinant human protein, FLJ18683, T3E, TCRE, CD3E, CD3-epsilon

More details

CDE-H5256-1K

Availability: within 7 days

1 870,00 €

Background
CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

Source
Recombinant Human CD3 epsilon, Fc,His Tag (CDE-H5256) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # NP_000724.1).
Predicted N-terminus: Asp 23

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 39.2 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh CD3E Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Distinct mutational profile and immune microenvironment in microsatellite-unstable and POLE-mutated tumors"
Hwang, Kim, Choi
J Immunother Cancer (2021) 9 (10)
(2) "Surface Ligand Valency and Immunoliposome Binding: when More Is Not Always Better"
Li, Di, Peng et al
Pharm Res (2021) 38 (9), 1593-1600
(3) "Acute TBK1/IKK-ε Inhibition Enhances the Generation of Disease-Associated Microglia-Like Phenotype Upon Cortical Stab-Wound Injury"
Rehman, Tar, Olamide et al
Front Aging Neurosci (2021) 13, 684171
Showing 1-3 of 851 papers.