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Recombinant Cynomolgus / Rhesus macaque IL-4 R alpha / CD124 Protein, 2 x 500µg  

Recombinant Cynomolgus / Rhesus macaque IL-4 R alpha / CD124 Protein, 2 x 500µg

Recombinant Cynomolgus / Rhesus macaque IL-4 R alpha, His Tag (ILR-C52H8) is expressed from human 293 cells (HEK293)

Synonym
Recombinant cynomolgus protein, IL4R,CD124,IL4RA

More details

ILR-C52H8-1K

Availability: within 7 days

1 980,00 €

Background
IL-4 is a pleiotropic cytokine produced by activated Th2 cells and mast cells, and plays a pivotal role in immune responses. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells. Hematopoietic cellular responses to IL-4 are mediated by a high affinity receptor complex comprised of the 140 kDa IL4Rα (CD124)subunit and the 70 kDa common cytokine γc chain (CD132). Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-­biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL­13Ra1 to form the type II receptor which is responsive to both IL­4 and IL­13. Interleukin-4 receptor has been shown to interact with SHC1.

Source
Cynomolgus / Rhesus macaque IL-4 R alpha, His Tag (ILR-C52H8) is expressed from human 293 cells (HEK293). It contains AA Met 26 - Arg 232 (Accession # G7Q0S7). In the region Met 26 - Arg 232, the AA sequence of Cynomolgus and Rhesus macaque IL-4 R alpha are homologus.
Predicted N-terminus: Met 26

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 25.6 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Bioactivity
Immobilized Human IL-4, Tag Free (Cat. No. IL4-H4218) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque IL-4 R alpha, His Tag (Cat. No. ILR-C52H8) with a linear range of 10-78 ng/mL (QC tested).

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Ikizawa K., et al., 2000, Biochem Biophys Res Commun, 268(1):54-9.
(2) Kashiwada M., et al., 2001, J Immunol, 167(11):6382-7.
(3) Hage T., et al., 1999, Cell 97 (2): 271–81.
(4) Gessner,A. et al., 2000, Immunobiology. 201 (3-4): 285-307.
(5) Wills­Karp, M., F.D. Finkelman, 2008, Sci. Signal. 1:pe55.