Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Cynomolgus / Rhesus macaque IL-4 R alpha / CD124 Protein, Fc Tag, 2 x500 µg  

Recombinant Cynomolgus / Rhesus macaque IL-4 R alpha / CD124 Protein, Fc Tag, 2 x500 µg

Recombinant Cynomolgus / Rhesus macaque IL-4 R alpha, Fc Tag (ILR-C5258) is expressed from human 293 cells (HEK293)

Synonym
Recombinant cynomolgus protein, IL4R,CD124,IL4RA

More details

ILR-C5258-1K

Availability: within 7 days

1 800,00 €

Background
IL-4 is a pleiotropic cytokine produced by activated Th2 cells and mast cells, and plays a pivotal role in immune responses. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells. Hematopoietic cellular responses to IL-4 are mediated by a high affinity receptor complex comprised of the 140 kDa IL4Rα (CD124)subunit and the 70 kDa common cytokine γc chain (CD132). Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-­biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL­13Ra1 to form the type II receptor which is responsive to both IL­4 and IL­13. Interleukin-4 receptor has been shown to interact with SHC1.

Source
Cynomolgus / Rhesus macaque IL-4 R alpha, Fc Tag (ILR-C5258) is expressed from human 293 cells (HEK293). It contains AA Met 26 - Arg 232 (Accession # G7Q0S7).
Predicted N-terminus: Met 26

Molecular Characterization
Cynomolgus IL-4 R alpha, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 50.4 kDa. The predicted N-terminus is Met 26 . The reducing (R) protein migrates as 60-90 kDa in SDS-PAGE .

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Ikizawa K., et al., 2000, Biochem Biophys Res Commun, 268(1):54-9.
(2) Kashiwada M., et al., 2001, J Immunol, 167(11):6382-7.
(3) Hage T., et al., 1999, Cell 97 (2): 271–81.
(4) Gessner,A. et al., 2000, Immunobiology. 201 (3-4): 285-307.
(5) Wills­Karp, M., F.D. Finkelman, 2008, Sci. Signal. 1:pe55.

The following products could also be interesting for you: