Recombinant human CD99 / MIC2 protein, Fc Tag, 100 µg

Background
CD99 antigen is also known as MIC2, single-chain type-1 glycoprotein, HBA71, MIC2X, MIC2Y, MSK5X, 12E7, which belongs to the CD99 family. CD99 / MIC2 is expressed on all leukocytes but highest on thymocytes. Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 / MIC2 plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. CD99 acts at the same site as, but independently of PECAM1. There is also experimental evidence that it binds to cyclophilin A.

Source
Recombinant human CD99 protein, Fc Tag (CD9-H5257) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 122 (Accession # AAH02584).
Predicted N-terminus: Asp 23

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 36.2 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Primary retroperitoneal perirenal CIC rearrangement sarcoma: A case report"
Yang, Liu, Huang
Oncol Lett (2022) 24 (3), 322
(2) "Human CD99L2 Regulates a Unique Step in Leukocyte Transmigration"
Rutledge, Ogungbe, Watson et al
J Immunol (2022)
(3) "Superficial myofibroblastoma of the lower female genital tract: A clinicopathological analysis of 15 cases"
Zhang, Shi, Zhou et al
Ann Diagn Pathol (2022) 60, 152010
Showing 1-3 of 2026 papers.