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Recombinant Cynomolgus Glypican 3 / GPC3 Protein, Fc Tag, 1 mg (2x 500µg)  

Recombinant Cynomolgus Glypican 3 / GPC3 Protein, Fc Tag, 1 mg (2x 500µg)

Recombinant Cynomolgus Glypican 3 / GPC3 Protein, Gln 25 - His 559, expressed from human 293 cells (HEK293), Fc Tag

Synonym: recombinant, cynomolgus, protein, GPC3, OCI5, Glypican-3, GTR2-2, MXR7, DGSX, SDYS, SGB, SGBS, SGBS1

More details

GP3-C5256-1K

Availability: within 7 days

1 800,00 €

Background
Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Source
Recombinant Cynomolgus Glypican 3, Fc Tag (GP3-C5256) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # XP_005594665.1). Predicted N-terminus: Gln 25 & Ser 359

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 49.4 kDa respectively. The protein migrates as 40 kDa, 60 kDa and 100-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity-ELISA
Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 2 μg/mL (100 μL/well) can bind Cynomolgus Glypican 3, Fc Tag (Cat. No. GP3-C5256) with a linear range of 1-31 ng/mL (QC tested).

Immobilized Cynomolgus Glypican 3, Fc Tag (Cat. No. GP3-C5256) at 0.5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GPC3 Antibody, Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).

References

(1)  Davoodi J., et al., 2007, Proteomics 7:2300-2310.
(2)  Veugelers M., et al., 2000, Hum. Mol. Genet. 9:1321-1328.

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